MarchMarch Experimental Objective: Evaluate the function of the GntR family transcription regulator ORF4 in the pseudomonad gene cluster and ultimately find an effective scheme to induce the expression of the mCherry fluorescent protein through promoter optimization.
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AprilApril Experimental Objective: Design a PX-specific riboswitch by modifying previously reported theophylline-specific riboswitch sequences for biosensor development.
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MayMay Experimental Objective: Summarize data from previous experiments and design a new biosensor—pYB1s-ndmDCEA.
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JuneJune Experimental Objective: Conduct site-saturation mutagenesis and screening.
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JulyJuly Experimental Objective: Perform site-directed saturation mutagenesis on key sites, followed by molecular docking and molecular dynamics simulation analysis.
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AugustAugust Experimental Objective: Optimize the promoter of NdmB^Q289T to increase PX yield.
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SeptemberSeptember Experimental Objective: Organize all experimental data during the period and begin writing the final report and creating the wiki.